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Commit c05b081b authored by DUGAT-BONY Eric's avatar DUGAT-BONY Eric
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Update file README.md

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## Viral metagenomic analysis
Raw data were decontaminated by removing reads originating from carrots, cabbage, and turnip, as well as low-quality reads, in order to assemble the reads into contigs. Contigs longer than 2 kb were selected, and viral contigs were identified.
All contigs larger than 2 kb were concatenated and dereplicated to retain only one vOTU. The cleaned reads were then mapped to the vOTUs to generate an abundance table of vOTUs in the samples.
The abundance table was processed using the Virome_analyses_2024.rmd script in RStudio to create phyloseq objects for DNA- and RNA-amplified samples.
### Reads QC
For each sample, raw reads were quality quality checked using [FastQC](https://github.com/s-andrews/FastQC) version 0.11.9, and the results were saved in `results/FastQC_report`. Here is the command line:
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