Raw sequence names
Sometimes Raw sequences filenames have format V4_515F_New_V4_806R_New-C14_GAGACTATGC_R2.fastq.
filformat for line 175 of dada2_fun.R
probably needs to be clarified.
for example, in this case line 175 should be : get.sample.name <- function(fname) strsplit(basename(fname), "_")[[1]][6]
and line 210 : sample.names <- sapply(strsplit(basename(fnFs), "_"), [
, 6)
Creating output directory: /media/pruiz/DATA/Philippe/Projets/Audrey/Anomaly/Bact/reads//filtered Error in filterAndTrim(fnFs, filtFs, fnRs, filtRs, truncLen = c(f_trunclen, : All output files must be distinct.