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Commit 2667a95a authored by Olivier Langella's avatar Olivier Langella
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phosphosislands tab added

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......@@ -369,6 +369,22 @@ If the expert output is enabled, the ODS output contains extra tabs :
\item ``peptide\_pos'' (see~\ref{ods_peptide_pos_tab})
\end{itemize}
\paragraph*{Phosphopeptide mode}
In this mode, only peptides containing one or more phosphorylation site are taken into account.
It builds a phosphoisland list. A phosphoisland is a merge of phosphopeptides sharing the same peptide sequence (possible overlaps). A phosphoisland could be present in one or several proteins : these phosphoislands are represented as subgroups.
The proteins sharing one or more subgroups of phosphoislands are represented by groups.
In this mode, the ODS report contains three tabs :
\begin{itemize}
\item ``phosphoislands'' (see~\ref{ods_phosphoisland_tab})
\item ``phosphopeptides'' (see~\ref{ods_phosphopeptide_tab})
\item ``compar\_Spectra'' (see~\ref{ods_phospho_compar_spectra_tab})
\end{itemize}
If the expert output is enabled, the ODS output contains extra tabs :
\begin{itemize}
\item ``spectra'' (see~\ref{ods_phospho_spectra_tab})
\end{itemize}
\subsection{Proteins tab (Fig~\ref{ods_individual_proteins})}
\label{ods_protein_tab}
\small
......@@ -555,6 +571,85 @@ This tab displays the protein abundance index (PAI) and the exponentially modifi
\end{figure}
\subsection{PhosphoIsland tab (Fig~\ref{ods_phospho_phosphoislands})}
\label{ods_phosphoisland_tab}
This tab displays the phosphoisland list.
\small
\begin{description}
\item[Group ID] Group ID of this phosphoisland : a group contains all proteins sharing one or more phosphoisland
\item[Sub-Group ID] Sub-Group of this phosphoisland : a subgroup contains all proteins sharing the same phosphopeptides
\item[PhosphoIsland ID] PhosphoIsland identifier : a set of phosphopeptides covering the same peptide sequence (possible overlaps) in a protein
\item [Description] The description of the phosphoisland's protein
\item [MW] The molecular weight of the phosphoisland's protein
\item [Phosphosites positions] the phopshorylation sites positions of the phosphoisland's phosphopeptides in this protein
\item [Spectra] Number of spectra identifying the phosphopeptides of this phosphoisland
\item [Uniques] Number of unique phosphopetide sequences
\item [number of proteins sharing these phosphosites] Number of proteins in this subgroup (sharing the same phosphoislands)
\end{description}
\normalsize
\begin{figure}[!ht]
\center \includegraphics[width=1.0\textwidth]{images/ods_phospho_phosphoislands}
\caption{phosphoislands results}
\label{ods_phospho_phosphoislands}
\end{figure}
\subsection{PhosphoPeptides tab (Fig~\ref{ods_phospho_phosphopeptides})}
\label{ods_phosphopeptide_tab}
This tab displays the protein abundance index (PAI) and the exponentially modified PAI (emPAI) by sample, for each top protein of a subgroup. This is convenient to have an idea of the presence/absence of a subgroup of proteins in a sample, and an indication about relative abundance in each samples.
\small
\begin{description}
\item[Group ID] Group ID of this protein
\item[Sub-Group ID] Sub-Group of this protein
\item[PhosphoIsland ID] Sub-Group of this protein
\end{description}
\normalsize
\begin{figure}[!ht]
\center \includegraphics[width=1.0\textwidth]{images/ods_phospho_phosphopeptides}
\caption{phosphopeptides results}
\label{ods_phospho_phosphopeptides}
\end{figure}
\subsection{Phosphopeptide mode compar Spectra tab (Fig~\ref{ods_phospho_compar_spectra})}
\label{ods_phospho_compar_spectra_tab}
This tab displays the protein abundance index (PAI) and the exponentially modified PAI (emPAI) by sample, for each top protein of a subgroup. This is convenient to have an idea of the presence/absence of a subgroup of proteins in a sample, and an indication about relative abundance in each samples.
\small
\begin{description}
\item[Group ID] Group ID of this protein
\item[Sub-Group ID] Sub-Group of this protein
\item[PhosphoIsland ID] Sub-Group of this protein
\end{description}
\normalsize
\begin{figure}[!ht]
\center \includegraphics[width=1.0\textwidth]{images/ods_phospho_compar_spectra}
\caption{compar\_Spectra results (mode phospho)}
\label{ods_phospho_compar_spectra}
\end{figure}
\subsection{Phosphopeptide mode Spectra tab (Fig~\ref{ods_phospho_spectra_expert})}
\label{ods_phospho_spectra_tab}
This tab displays the protein abundance index (PAI) and the exponentially modified PAI (emPAI) by sample, for each top protein of a subgroup. This is convenient to have an idea of the presence/absence of a subgroup of proteins in a sample, and an indication about relative abundance in each samples.
\small
\begin{description}
\item[Group ID] Group ID of this protein
\item[Sub-Group ID] Sub-Group of this protein
\item[PhosphoIsland ID] Sub-Group of this protein
\end{description}
\normalsize
\begin{figure}[!ht]
\center \includegraphics[width=1.0\textwidth]{images/ods_phospho_spectra_expert}
\caption{Spectra results (mode phospho)}
\label{ods_phospho_spectra_expert}
\end{figure}
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