upgrade sample handling

bin/ng6_database.sql

@@ -1003,8 +1003,15 @@ CREATE TABLE IF NOT EXISTS `tx_nG6_sample` (
   `deleted` tinyint(4) NOT NULL DEFAULT '0',
   `hidden` tinyint(4) NOT NULL DEFAULT '0',
   `run_id` int(11) NOT NULL DEFAULT '0',
-  `mid` varchar(255) NOT NULL DEFAULT '',
+  `sample_id` varchar(255) NOT NULL DEFAULT '',
+  `name` varchar(255) NOT NULL DEFAULT '',
   `description` varchar(255) NOT NULL DEFAULT '',
+  `reads1` text NOT NULL DEFAULT '',
+  `reads2` text NOT NULL DEFAULT '',
+  `type` varchar(255) NOT NULL DEFAULT '',
+  `insert_size` int(11) NOT NULL DEFAULT '0',
+  `species` varchar(255) NOT NULL DEFAULT '',
+  `nb_sequence` int(11) NOT NULL DEFAULT '0',
   PRIMARY KEY (`uid`),
   KEY `parent` (`pid`)
 ) ENGINE=InnoDB DEFAULT CHARSET=utf8 AUTO_INCREMENT=1 ;

src/ng6/analysis.py

@@ -330,7 +330,7 @@ class Analysis (Component):
     def _create_and_archive(self, files, archive_name=None, prefix="dir"):
         """
         return the web path to the archive files
-        If there are mids, datas will be organised by mids
+        If there are samples, datas will be organised by samples
           @param files        : table of file
           @param archive_name : the archive name to use, if not set will be
                                 the same as link_value
@@ -352,14 +352,14 @@ class Analysis (Component):
         #If some files have the same name
         if len(file_basenames) != len(set(file_basenames)) :
 
-            try : # If there are mids
+            try : # If there are samples
 
                 if self.run :
-                    mids_desc = self.run.get_mids_description()
+                    samples = self.run.get_samples()
                 else :
                     run_id = self.__get_run_id()
                     my_run = Run.get_from_id(run_id)
-                    mids_desc = my_run.get_mids_description()
+                    samples = my_run.get_samples()
                 gfiles = {}
                 ungrouped_files = []
                 what_left = []
@@ -367,16 +367,17 @@ class Analysis (Component):
                     ungrouped_files.append(file)
                     what_left.append(file)
 
-                # First group files if they have the mid name in their path
+                # First group files if they have the sample name in their path
                 for file in files :
 
                     # In case of multiple description take the longuest
                     best_description = []
-                    for mid_desc in mids_desc.keys():
-                        mid_desc_regex = re.compile(".*" + mid_desc + ".*")
-                        mdr = mid_desc_regex.match(file)
-                        if mdr != None:
-                            best_description.append(mid_desc)
+                    for sample in samples :
+                        spname = sample.name
+                        spname_regex = re.compile(".*" + spname + ".*")
+                        spr = spname_regex.match(file)
+                        if spr :
+                            best_description.append(spname)
 
                     max_len = 0
                     final_description = None

src/ng6/ng6workflow.py

@@ -32,7 +32,7 @@ from ng6.run import Run
 from ng6.sample import Sample
 from ng6.utils import Utils
 
-from workflows.types import adminlogin, casava_dir
+from workflows.types import adminlogin, casava_dir, existingproject
 
 class BasicNG6Workflow (Workflow):
 
@@ -46,7 +46,7 @@ class BasicNG6Workflow (Workflow):
 
     def get_parameters_per_groups(self, parameters_section="parameters"):
         name, description, parameters = self._get_from_config(parameters_section)
-        parameters.extend(self._get_required_parameters())
+        parameters.extend(self._required_parameters)
         pgparameters, parameters_order = {}, []
         for param in parameters:
             if param.group not in parameters_order: parameters_order.append(param.group)
@@ -55,9 +55,6 @@ class BasicNG6Workflow (Workflow):
             else:
                 pgparameters[param.group] = [param]
         return [pgparameters, parameters_order]
-    
-    def _get_required_parameters(self):
-        return self._required_parameters
 
     def add_component(self, component_name, args=[], kwargs={}, component_prefix="default", parent=None, addto="run"):
         # first build and check if this component is OK
@@ -119,20 +116,20 @@ class NG6Workflow (BasicNG6Workflow):
 
     def __init__(self, args={}, id=None, function= "process", parameters_section="parameters"):
         BasicNG6Workflow.__init__(self, args, id, function, parameters_section)
-        self.samples = []
-        self._required_parameters.extend(self._add_required_parameters())
+        self._required_parameters.extend(self._add_run_parameters())
         self._required_parameters.extend(self._add_sample_parameters())
-        self.parameters.extend(self._add_required_parameters())
+        self.parameters.extend(self._add_run_parameters())
         self.parameters.extend(self._add_sample_parameters())
         self.args = self._extend_and_format_args(self.parameters, args)
         
-        self.__all_reads1__ = []
-        self.__all_reads2__ = []
-        self.__samples_name__ = []
+        self.samples = []
+        self.reads1 = []
+        self.reads2 = []
+        self.samples_names = []
 
-    def _add_required_parameters(self):
+    def _add_run_parameters(self):
         orequired = []
-        orequired.append(Parameter("project_id", "The project id the run belongs to", "--project-id", required=True, type=int, group="Run information"))
+        orequired.append(Parameter("project_id", "The project id the run belongs to", "--project-id", required=True, type=existingproject, group="Run information"))
         orequired.append(Parameter("run_name", "Give a name to your run", "--name", required=True, group="Run information", display_name="Name"))
         orequired.append(Parameter("run_description", "Give a description to your run", "--description", required=True, group="Run information", display_name="Description"))
         orequired.append(Parameter("run_date", "When were the data produced","--date",  required=True, type=date, group="Run information", display_name="Date"))
@@ -145,51 +142,62 @@ class NG6Workflow (BasicNG6Workflow):
     def _add_sample_parameters(self):
         osamples = []
         subparams = []
-        subparams.append(Parameter("sample_name", "The name of the sample, MID for 454 data", "sample_name"))
+        subparams.append(Parameter("sample_id", "The uniq identifier of the sample", "sample_id"))
+        subparams.append(Parameter("sample_name", "A descriptive name for the sample", "sample_name"))
         subparams.append(Parameter("sample_description", "A brief description of the sample", "sample_description" ))
         subparams.append(Parameter("read1", "Read 1 data file path", "read1", required=True, type = "localfile", action= "append" ))
         subparams.append(Parameter("read2", "Read 2 data file path", "read2", type = "localfile", action="append" ))
+        subparams.append(Parameter("type", "Read orientation and type", "type", choices = '|'.join(Sample.AVAILABLE_TYPES) ))
+        subparams.append(Parameter("insert_size", "Insert size for paired end reads", "insert_size", type ='int' ))
+        subparams.append(Parameter("species", "Species related to this sample", "species"))
+        subparams.append(Parameter("nb_sequence", "Number of sequences in the sample file", "nb_sequence", type =  'int'))
         osamples.append(Parameter("sample", "Definition of a sample", "--sample", action = "append", type="multiple", required = True, sub_parameters = subparams, group = "exclude-sample"  ))
         return osamples
     
-    def __preprocess_samples__(self):
-        if not (self.args.has_key("sample") and self.args['sample']):
-            sys.stderr.write( "--sample option is required\n" )
-            sys.exit()
-        
-        mids_description = {}
+    def __create_samples__(self):
         for sd in self.args['sample'] :
-            sp_object = Sample(sd['sample_name'], sd['sample_description'], sd['read1'], sd['read2'])
-            if sp_object.name and sp_object.description :
-                mids_description[sp_object.name] = sp_object.description
-            if sp_object.name :
-                self.__samples_name__.append(sp_object.name)
-            
-            self.__all_reads1__.extend(sp_object.reads1)
-            self.__all_reads2__.extend(sp_object.reads2)
-            
+            sp_object = Sample( sd['sample_id'], sd['read1'], sd['read2'], name = sd['sample_name'], description = sd['sample_description'], type = sd['type'], 
+                               insert_size = sd['insert_size'], species = sd['species'], nb_sequence = sd['nb_sequence'] )
+            self.reads1.extend(sp_object.reads1)
+            self.reads2.extend(sp_object.reads2)
             self.samples.append(sp_object)
-        
-        if mids_description :
-            self.runobj.add_mids_description(mids_description)
-    
-    def get_samples(self):
-        return self.samples
     
-    def get_all_reads1(self):
-        return self.__all_reads1__
+    def __preprocess_samples(self):
+        samples_ids = []
+        pidx = 1
+        nidx = 1
+        for index, sample in enumerate(self.samples) :
+            if sample.name :
+                self.samples_names.append(sample.name)
+            else :
+                sample.name = 'SAMPLE_' + str(nidx)
+                nidx += 1
+                
+            if sample.sample_id :
+                if sample.sample_id in samples_ids :
+                    sys.stderr.write( "Sample identifier %s must be uniq ( sample %s ).\n" % sample.sample_id, sample.name )
+                    sys.exit(1)
+            # ad an automatic id for samples
+            else :
+                sample.sample_id = 'sample_' + str(pidx)
+                pidx += 1
+            samples_ids.append(sample.sample_id)
         
-    def get_all_reads2(self):
-        return self.__all_reads2__
-    
-    def get_nospace_runname(self):
-        return "_".join(self.runobj.name.split())
-    
-    def get_samples_name(self):
-        return self.__samples_name__
+        
+        if len(self.samples_names) != 0 :
+            if len(self.samples_names) != len (self.samples) :
+                sys.stderr.write( "All samples must have a defined sample name" )
+                sys.exit(1)
+          
+    def get_all_reads(self, type = None):
+        if type in ['R1', 'r1', 'read1', 1, '1'] :
+            return self.reads1
+        elif type in ['R2', 'r2', 'read2', 2, '2'] :
+            return self.reads2
+        return self.reads1 + self.reads2
     
     def is_paired_end(self):
-        return len(self.get_all_reads2()) > 0
+        return len(self.reads2) > 0
     
     def pre_process(self):
         # start from an existing project
@@ -208,11 +216,10 @@ class NG6Workflow (BasicNG6Workflow):
         self.project.add_run(self.runobj)
         self.metadata.append("run_id="+str(self.runobj.id))
         
-        self.__preprocess_samples__()
-        
-        if not self.samples :
-            sys.stderr.write( "Please define at least one sample.\n" )
-            sys.exit()
+        self.__create_samples__()
+        self.__preprocess_samples()
+        # add samples to run
+        self.runobj.add_samples(self.samples)
         
     def post_process(self):
         # once everything done, sync directories
@@ -230,10 +237,10 @@ class CasavaNG6Workflow(NG6Workflow):
         self.parameters.extend(self._add_casava_parameters())
         self.args = self._extend_and_format_args(self.parameters, args)
         
-        self.__is_casava__ = False
-        self.__group_prefix__ = None
-        self.__undetermined_reads1__ = []
-        self.__undetermined_reads2__ = []
+        self.is_casava = False
+        self.group_prefix = None
+        self.undetermined_reads1 = []
+        self.undetermined_reads2 = []
     
     def _add_casava_parameters(self):
         params = []
@@ -243,23 +250,11 @@ class CasavaNG6Workflow(NG6Workflow):
         params.append(Parameter("casava", "Definition of a casava directory for sample retrieving", "--casava", required = True, sub_parameters = subparams, type = "multiple", group = "exclude-sample"  ))
         return params
     
-    def __preprocess_samples__(self):
+    def __create_samples__(self):
         if self.args.has_key("casava") and self.args["casava"] and self.args["casava"]['casava_directory'] and self.args["casava"]['lane']:
             self.__preprocess_casavadir(self.args["casava"]['casava_directory'], '_'.join( self.project.get_name().split() ), self.args["casava"]['lane'])
         else :
-            NG6Workflow.__preprocess_samples__(self)
-    
-    def get_all_undetermined_read1(self):
-        return self.__undetermined_reads1__
-    
-    def get_all_undetermined_read2(self):
-        return self.__undetermined_reads1__
-    
-    def is_casava(self):
-        return self.__is_casava__
-    
-    def get_group_prefix(self):
-        return self.__group_prefix__
+            NG6Workflow.__create_samples__(self)
     
     def __preprocess_casavadir(self, casava_directory, project_name, lane_number):
         """
@@ -268,79 +263,66 @@ class CasavaNG6Workflow(NG6Workflow):
             @param project_name : files in each sample are part of this project
             @param lane_number : files in each sample are sequenced on this lane
         """
-        self.__is_casava__ = True
-        samples = []
-        mids_description = {}
+        self.is_casava = True
+        
         with open(os.path.join(casava_directory, "SampleSheet.mk")) as fh :
+            barcodes_list = []
+            sample_ids_list = []
+            subdirs_list = []
             for line in fh :
                 if line.startswith("l" + lane_number + "_BARCODES"):
                     parts = line.strip().split(":=")
-                    barcodes_list = parts[1].split(" ")
-                    for i in range(len(barcodes_list)):
-                        samples.append({'barcode':barcodes_list[i]})
-                # Retrieve samples ids
+                    barcodes_list = parts[1].split()
                 elif line.startswith("l" + lane_number + "_SAMPLEIDS" ):
                     parts = line.strip().split(":=")
                     sample_ids_list = parts[1].split(" ")
-                    for i in range(len(sample_ids_list)):
-                        samples[i]['sample_id'] = sample_ids_list[i]
-                # Retrieve folder          
                 elif line.startswith("l" + lane_number + "_SUBDIRS"):
                     parts = line.strip().split(":=")
                     subdirs_list = parts[1].split(" ")
-                    for i in range(len(subdirs_list)):
-                        samples[i]['subdir'] = subdirs_list[i]
-        
-        # Filter on project name 
-        aux_samples = []
-        for sample in samples:
-            if (re.match("Project_" + project_name + "/Sample_.+", sample['subdir']) is not None) or (sample['subdir'].startswith("Undetermined_indices")):
-                aux_samples.append(sample)
-        samples = aux_samples
-        
-        if len(samples) == 0:
-            raise ValueError, "The project '" + project_name + "' in lane '" + lane_number + "' doesn't exist in CASAVA directory " + casava_directory 
-        
-        for sample in samples:
-            # Write line in the index description
-            if (sample['barcode'] != "NoIndex") and (not sample['subdir'].startswith("Undetermined_indices")) :
-                mids_description[sample['barcode']] = sample['sample_id']
             
-            read1_files = []
-            read2_files = []
-            undetermined_read1_files = []
-            undetermined_read2_files = []
-            # Write files lists
-            for file in os.listdir(casava_directory + "/" + sample['subdir']):
-                if file.endswith(".fastq.gz") and re.search(".*_L00" + lane_number + "_.*", file):
-                    if re.search(".*_R1_.*", file):
-                        if not sample['subdir'].startswith("Undetermined_indices"):
-                            read1_files.append(casava_directory + "/" + sample['subdir'] + "/" + file)
-                        else:
-                            undetermined_read1_files.append(casava_directory + "/" + sample['subdir'] + "/" + file)
-                    if re.search(".*_R2_.*", file):
-                        if not sample['subdir'].startswith("Undetermined_indices"):
-                            read2_files.append(casava_directory + "/" + sample['subdir'] + "/" + file)
-                        else:
-                            undetermined_read2_files.append(casava_directory + "/" + sample['subdir'] + "/" + file)
+            assert  len(barcodes_list) == len(sample_ids_list) == len(subdirs_list), "Invalid lane {0} for project {1} in SampleSheet.mk".format(lane_number,project_name)
             
-            sp_object = Sample(sample['sample_id'], sample['sample_id'], read1_files, read2_files)
-            sp_object.add_metadata('undetermined_read1_files', undetermined_read1_files)
-            sp_object.add_metadata('undetermined_read2_files', undetermined_read2_files)
-            sp_object.add_metadata('barcode', sample['barcode'])
-            sp_object.add_metadata('is_casava', True)
-            if sp_object.name :
-                self.__samples_name__.append(sp_object.name)
+            # parse samples
+            for i in range(len(barcodes_list)):
+                read1_files = []
+                read2_files = []
+                undetermined_read1_files = []
+                undetermined_read2_files = []
                 
-            self.__all_reads1__.extend(read1_files)
-            self.__all_reads2__.extend(read2_files)
-            self.__undetermined_reads1__.extend(undetermined_read1_files)
-            self.__undetermined_reads2__.extend(undetermined_read2_files)
-            self.samples.append(sp_object)
-            
-        if mids_description :     
-            self.runobj.add_mids_description(mids_description)
-        
+                sample = {
+                    'barcode'   : barcodes_list[i],
+                    'sample_id' : sample_ids_list[i],
+                    'subdir'    : subdirs_list[i]
+                }
+                
+                # filter on project name
+                if re.match("Project_" + project_name + "/Sample_.+", sample['subdir']) or sample['subdir'].startswith("Undetermined_indices"):
+                    for file in os.listdir(casava_directory + "/" + sample['subdir']):
+                        filepath = casava_directory + "/" + sample['subdir'] + "/" + file
+                        if file.endswith(".fastq.gz") and re.search(".*_L00" + lane_number + "_.*", file):
+                            if re.search(".*_R1_.*", file):
+                                if not sample['subdir'].startswith("Undetermined_indices"):
+                                    read1_files.append(filepath)
+                                else:
+                                    undetermined_read1_files.append(filepath)
+                            if re.search(".*_R2_.*", file):
+                                if not sample['subdir'].startswith("Undetermined_indices"):
+                                    read2_files.append(filepath)
+                                else:
+                                    undetermined_read2_files.append(filepath)
+                    
+                    self.reads1.extend(read1_files)
+                    self.reads2.extend(read2_files)
+                    self.undetermined_reads1.extend(undetermined_read1_files)
+                    self.undetermined_reads2.extend(undetermined_read2_files)
+                    
+                    # No sample if undetermined
+                    if not sample['subdir'].startswith("Undetermined_indices") :
+                        sp_object = Sample(sample['barcode'], sample['sample_id'], read1_files, read2_files)
+                        sp_object.add_metadata('undetermined_read1_files', undetermined_read1_files)
+                        sp_object.add_metadata('undetermined_read2_files', undetermined_read2_files)
+                        sp_object.add_metadata('barcode', sample['barcode'])
+                        sp_object.add_metadata('is_casava', True)
+                
+        self.group_prefix = (Utils.get_group_basenames(self.get_all_reads(), "read")).keys()
         
-        self.__group_prefix__ = (Utils.get_group_basenames(self.get_all_reads1()+self.get_all_reads2(), "read")).keys()
-        
\ No newline at end of file

src/ng6/run.py

@@ -64,6 +64,7 @@ class Run(object):
         self.description = description
         self.sequencer = sequencer
         self.__mids_description = None
+        self.__samples = None
         self.raw_files = []
         self.admin_login = None
         # Set the temp folder to the ng6 temp folder
@@ -107,38 +108,6 @@ class Run(object):
         self.set_full_size(full_size)
         self.archive_files(self.raw_files, mode)
     
-    def export_mids_to_newbler_cfg_file(self, error=2, midscheme="454MIDS"):
-        """
-        Export the config file in newbler format
-        the script add_raw_files.
-          @param files: the files to archive
-          @param mode: can be none, gz, bz2, tar.gz and tar.bz2    
-        """
-        mids_cfg_path = tempfile.NamedTemporaryFile(suffix=".cfg").name
-        mid_file = open(mids_cfg_path, "w")
-        mid_file.write(midscheme+"\n")
-        mid_file.write("{\n")
-        # MID1:desc_ex1;MID2:desc_ex2;MID3,MID4:
-        cfg_reader = NG6ConfigReader()
-        mids = cfg_reader.get_454_mids()
-        # First write the run mids config file
-        for mid in self.__mids_description:
-            for mid_name in mid.split(","):
-                try:
-                    mid_name = mid_name.strip()
-                    seq = mids[mid_name.lower()].split(",")
-                    seq_val = ''
-                    if len(seq) > 1:
-                        seq_val += '"' + seq[0] + '", ' + str(error) + ', "' + seq[1] + '";'
-                    else:
-                        seq_val += '"' + seq[0] + '", ' + str(error) + ';'
-                    mid_file.write('\tmid = "' + self.__mids_description[mid] + '", ' + seq_val +"\n")
-                except:
-                    pass
-        mid_file.write("}")
-        mid_file.close()
-        return [mids_cfg_path, midscheme]
-    
     def archive_files(self, files, mode, archive_name="ng6_archive.tar", delete=False):
         """
         Copy, archive and compress the files list to the run. Files can then be downloaded and gave back by
@@ -236,7 +205,7 @@ class Run(object):
             logging.getLogger("Run.sync").error("Error when synchronizing analysis that belongs to run id=" + str(self.id))
             raise Exception(str(err))
         # If a sync is required
-        if len(os.listdir(self.__get_work_directory())):
+        if os.path.exists(self.__get_work_directory()) and len(os.listdir(self.__get_work_directory())):
             # Finally the Run itself
             try :
                 # not created for updates
@@ -278,32 +247,43 @@ class Run(object):
         """
         return self.__get_save_directory()
 
-    def get_mids_description(self):
-        """
-        Return a dictionary with mids description
-          @return : {"MID1":"mid1_desc", "MID2":"mid2_desc", ...}
-        """
-        if self.__mids_description :
-            return self.__mids_description
+    def get_samples(self):
+        '''
+            return a list of samples object
+        '''
+        if self.__samples :
+            return self.__samples
         else :
             t3mysql = t3MySQLdb()
-            return t3mysql.select_run_samples(self.id)
-
-    def add_mids_description(self, mids_desc):
-        """
-        Add a mids description to the run
-          @param mids_desc  : the mids description dictionnary
-                              {"MID1":"mid1_desc", "MID2":"mid2_desc", ...}
-        """
-        # First add the description to the python object
-        self.__mids_description = mids_desc
+            results = t3mysql.select_run_samples(self.id)
+            samples = []
+            for res in results :
+                samples.append(Sample( res['sample_id'], res['reads1'], reads2 = res['reads2'], name = res['name'], description = res['description'], 
+                                       type = res['type'], insert_size = res['insert_size'], species = res['species'], nb_sequence = res['nb_sequence'] ))
+            return samples
         
-        if self.id != None:
+    
+    def add_samples(self, samples):
+        '''
+            Add a list of samples to the run
+            @param samples: the list of samples object to add
+        '''
+        self.__samples = samples
+        if self.id != None :
             # Update database
             t3mysql = t3MySQLdb()
-            for mid_name in self.__mids_description.keys():
-                t3mysql.add_sample_to_run(self.id, mid=mid_name, description=self.__mids_description[mid_name])
-
+            for spo in self.__samples:
+                reads1 = []
+                reads2 = []
+                if spo.reads1:
+                    reads1 = [ os.path.basename(ff) for ff in spo.reads1 ]
+                if spo.reads2 :
+                    reads2 = [ os.path.basename(ff) for ff in spo.reads2 ]
+                                
+                t3mysql.add_sample_to_run(self.id, sample_id = spo.sample_id, reads1 = reads1, reads2 = reads2, name=spo.name, 
+                                          description = spo.description, type = spo.type, insert_size = spo.insert_size, 
+                                          species = spo.species, nb_sequence = spo.nb_sequence)
+    
     def get_config_file(self):
         """
         Return a Run config file
@@ -370,11 +350,12 @@ class Run(object):
         self.id = t3mysql.add_run(self.name, self.date, self.directory, self.species, self.data_nature,
                                   self.type, 0, 0, self.description, self.sequencer, self.admin_login)
         
-        # If there is some mids desc provided
-        if self.__mids_description != None:
-            for mid_name in self.__mids_description.keys():
-                t3mysql.add_sample_to_run(self.id, mid=mid_name, description=self.__mids_description[mid_name])
-
+        # if there are samples
+        if self.__samples :
+            for spo in self.__samples:
+                t3mysql.add_sample_to_run(self.id, name=spo.name, description=spo.description, reads1 = spo.reads1, 
+                                          reads2 = spo.reads2, type = spo.type, insert_size = spo.insert_size, 
+                                          species = spo.species, nb_sequence = spo.nb_sequence)
         # Finaly return it's id
         return self.id
 

src/ng6/sample.py

@@ -18,15 +18,34 @@
 
 class Sample(object):
     
-    def __init__(self, name, description, reads1, reads2 = None):
+    AVAILABLE_TYPES = ["pe", "se", "ose", "ope", "mp"]
+    
+    def __init__(self, sample_id, reads1, reads2 = None, name = None, description = None, type = None, insert_size = None, species = None, nb_sequence = None ):
+        self.sample_id = sample_id
         self.name = name
         self.description = description
         self.reads1 = reads1
+        self.reads2 = reads2
+        self.insert_size = insert_size
+        self.nb_sequence = nb_sequence
+        self.species = species
+        self.type = type
+        
         if isinstance(reads1, str) :
             self.reads1 = [reads1]
-        self.reads2 = reads2
+        
         if isinstance(reads2, str) :
             self.reads2 = [reads2]
+        
+        if self.type is None:
+            if self.reads2 :
+                self.type = self.AVAILABLE_TYPES[0]
+            else :
+                self.type = self.AVAILABLE_TYPES[1]
+        
+        if self.nb_sequence and isinstance(self.nb_sequence, str) :
+            self.nb_sequence = int(self.nb_sequence)
+        
         self.metadata = {}
         
     def add_metadata(self, key, value):
@@ -39,3 +58,5 @@ class Sample(object):
     def has_metadata(self, key):
         return self.metadata.has_key(key)
     
+    
+

src/ng6/t3MySQLdb.py

@@ -438,21 +438,52 @@ class t3MySQLdb(object):
         conn.close()
         return analysis_ids
 
-    def add_sample_to_run(self, run_id, mid, description):
+    def add_sample_to_run(self, run_id, sample_id, reads1, reads2 = None, name = None , description = None,