Run_stats.tpl 14.8 KB
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{*
Copyright (C) 2009 INRA
 
This program is free software: you can redistribute it and/or modify
it under the terms of the GNU General Public License as published by
the Free Software Foundation, either version 3 of the License, or
(at your option) any later version.

This program is distributed in the hope that it will be useful,
but WITHOUT ANY WARRANTY; without even the implied warranty of
MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
GNU General Public License for more details.

You should have received a copy of the GNU General Public License
along with this program.  If not, see <http://www.gnu.org/licenses/>.
*}

{extends file='AnalysisTemplate.tpl'}
{block name=params}
	{assign var="params" value=" "|explode:$analyse.params}
	{foreach from=$analyse_results key=sample item=sample_results}
	{assign var="sample" value=$sample}
	{/foreach}
	<ul>
		{if in_array("minSubReadLength", $params)}
		{assign var="minSubReadLength" value=$params|@array_keys:"minSubReadLength"}
		<li class="parameter">Subreads shorter than {$params[$minSubReadLength[0]+1]} (in base pairs) are filtered out and excluded from analysis.</li>
		{/if}
		{if in_array("readScore", $params)}
		{assign var="polymerase_read_qual" value=$params|@array_keys:"readScore"}
		<li class="parameter">Polymerase reads with lower quality than {$params[$polymerase_read_qual[0]+1]} are filtered out and excluded from analysis. </li>
		{/if}
		{if in_array("minLength", $params)}
		{assign var="polymerase_read_length" value=$params|@array_keys:"minLength"}
		<li class="parameter">Polymerase reads shorter than {$params[$polymerase_read_length[0]+1]} (in base pairs) are filtered out and excluded from analysis.</li>
		{/if}
		{if in_array("barcode_file", $params)}
		{assign var="barcode_file" value=$params|@array_keys:"barcode_file"}
		<li class="parameter">Input barcode file : {$params[$barcode_file[0]+1]}.</li>
		{/if}
		{if in_array("barcode_score", $params)}
		{assign var="barcode_score" value=$params|@array_keys:"barcode_score"}
		<li class="parameter">Min identical base for barcode : {$params[$barcode_score[0]+1]}.</li>
		{/if}
	</ul>
{/block}


{block name=results_title} Reports {/block}
{block name=results}
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	{assign var="analyse_results_sorted" value=$analyse_results["ONT_sample"]|@ksort}
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	{assign var="metrics" value=$analyse_results["metrics"]}
	{assign var="basic" value=$analyse_results["ONT_sample"].basic}
	{assign var="quality" value=$analyse_results["ONT_sample"].quality}
	{assign var="plots" value=$analyse_results["ONT_sample"].plots}
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	{assign var="plots_barcode" value=$analyse_results["ONT_sample"].plots_barcode}
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	{assign var='basic_headers' value=','|explode:$metrics['basic'].headers}
	{assign var='basic_count' value=$basic_headers|@count}
    
	{assign var='quality_headers' value=','|explode:$metrics['quality'].headers}
	{assign var='quality_count' value=$quality_headers|@count}
	
	{assign var='plots_headers' value=','|explode:$metrics['plots'].headers}
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	{assign var='plots_count' value=$plots_headers|@count}
	
	{assign var='plots_barcode_headers' value=','|explode:$metrics['plots_barcode'].headers}
	{assign var='plots_barcode_count' value=$plots_barcode_headers|@count}
    
	{assign var='barcode_headers' value=','|explode:$metrics['barcode'].headers|@ksort}
	{assign var='barcode_headers_count' value=$barcode_headers|@count}
    {assign var='barcode_name_sample' value=','|explode:$metrics['barcode'].names|@ksort}
    {assign var='barcode_name_count' value=$barcode_name_sample|@count}
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    {*debug*}
	<div>
		<legend>Analyse results report - Sample name : {$descriptions.sample_1}</legend>
		
		{assign var='data_col' value=2}
		<div class="row">
			{if $metrics['basic']}
				<div class="col-md-4 col-lg-4">
					<table id="ont_table_basic" class="table table-striped table-bordered dataTable analysis-result-table">
					<thead><tr><th colspan="2"><h4>Basic matrics report</h4></thead></tr></th>
						<tbody>
						{foreach from=$basic_headers key=k item=head}
							<tr>
								{if ($head == 'median_yield_per_sec')  or ($head == 'mean_yield_per_sec')}
									<td><label>{$head}</label></td>
									<td>{($basic.$head)|number_format:2:',':' '}</td>
								{else}
									<td><label>{$head}</label></td>
									<td>{($basic.$head)|number_format:0:',':' '}</td>
								{/if}
							</tr>
							{$data_col = $data_col + 1}
						{/foreach}
						</body>
					</table>
				</div>
			{/if}
			{if $metrics['quality']}
				<div class="col-md-4 col-lg-4">
					<table id="ont_table_quality" class="table table-striped table-bordered dataTable analysis-result-table">
						<thead><tr><th colspan="2"><h4>Quality report</h4></thead></tr></th>
					<tbody>
						{foreach from=$quality_headers key=k item=head}
							<tr>
								{if ($head == 'median_read_quality')  or ($head == 'mean_read_quality')}
									<td><label>{$head}</label></td>
									<td>{($quality.$head)|number_format:2:',':' '}</td>
								{elseif $head|strstr:"nb_read_Q"}
									<td><label>{$head}</label></td>
									<td>{($quality.$head)|number_format:0:',':' '} ({($quality.$head / $basic.nb_reads*100)|number_format:2:',':' '}%)</td>
								{else}
									<td><label>{$head}</label></td>
									<td>{($quality.$head)|number_format:0:',':' '} ({($quality.$head / $basic.total_bases*100)|number_format:2:',':' '}%)</td>
								{/if}
							</tr>
							{$data_col = $data_col + 1}
						{/foreach}
					</tbody>
					</table>
				</div>
			{/if}
			{if $metrics['plots']}
				<div class="col-md-3 col-lg-3">
					<table id="ont_table_plot" class="table table-striped table-bordered dataTable analysis-result-table">
						<thead><tr><th colspan="2"><h4>Plots report</h4></h4></thead></tr></th>
					<tbody>
						{foreach from=$plots_headers key=k item=head}
							<tr>
									<td><label>{$head}</label></td>
									<td><a class="imglink" href="{$plots.$head}" ><i class="glyphicon glyphicon-picture"></i></a></td>
							</tr>
							{$data_col = $data_col + 1}
						{/foreach}
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						{if $barcode_name_count > 1 }
						{foreach from=$plots_barcode_headers key=k item=head}
							<tr>
									<td><label>{$head}</label></td>
									<td><a class="imglink" href="{$plots_barcode.$head}" ><i class="glyphicon glyphicon-picture"></i></a></td>
							</tr>
							{$data_col = $data_col + 1}
						{/foreach}
						{/if}
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					</tbody>
					</table>
				</div>
			{/if}
		</div>
	</div>
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	{*If there are barcodes file in the run*}
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	{if $barcode_name_count > 1 }	
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	<table id="ont_stats_datatable" class="table table-striped table-bordered dataTable analysis-result-table">
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		<thead>
			<tr>
				<th rowspan="2"><center><input type="checkbox" id="chk_all_sample"/></center></th>
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				{assign var="nb_samples" value=$barcode_name_count}
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				<th class="string-sort" rowspan="2" id="th_id_1"><center>Sample {if $nb_samples > 1 }({$nb_samples}){/if}</center></th>
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        		<th colspan="{$barcode_headers_count}"><center>Barcode ONT results</center></th>
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			</tr>
			<tr>
				{assign var="th_id" value=2}
				{foreach from=$barcode_headers key=k item=head}
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					{if $head == 'total_bases'}
						<th class = "numeric-sort" id="th_id_{$th_id}">Total bases</th>
					{elseif $head == 'nb_reads'}
						<th class = "numeric-sort"  id="th_id_{$th_id}">Nb reads</th>
					{elseif $head == 'median_read_length'}
						<th class = "numeric-sort"  id="th_id_{$th_id}">Median read length</th>
					{elseif $head == 'median_read_quality'}
						<th class = "numeric-sort"  id="th_id_{$th_id}">Median read quality</th>
					{elseif $head == 'N50_read_length'}
						<th class = "numeric-sort"  id="th_id_{$th_id}">N50 read length</th>
					{elseif $head == 'L50_read_length'}
						<th class = "numeric-sort"  id="th_id_{$th_id}">L50 read length</th>
					{elseif $head == 'barcode_score'}
						<th class = "numeric-sort"  id="th_id_{$th_id}">Barcode score</th>
					{elseif $head == 'nb_read_Q>10'}
						<th class = "numeric-sort"  id="th_id_{$th_id}">Nb reads Q>10</th>
					{elseif $head == 'total_bases_Q>10'}
						<th class = "numeric-sort"  id="th_id_{$th_id}">Total bases Q>10</th>
					{elseif $head == 'median_yield_per_sec'}
						<th class = "numeric-sort"  id="th_id_{$th_id}">Median yield per sec</th>
					{elseif $head == 'nb_actif_channel'}
						<th class = "numeric-sort"  id="th_id_{$th_id}">Nb actif channel</th>
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					{/if}
					{$th_id = $th_id +1}
				{/foreach}
			</tr>
		</thead>
		<tbody>
			{$i = 1}
			{assign var="totalReads" value=0}
			{assign var="totalBases" value=0}
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            {foreach from=$analyse_results_sorted key=sample item=sample_results}
            	{if $sample|in_array:$barcode_name_sample}
            		<tr>
						<td><center><input type="checkbox" id="chk_sample_{$i}" value="sample"/></center></td>
						<td id='sample_{$i}_col_1' class="sample_name">{$sample}</td>
						{$col_id = 2}
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						{foreach from=$barcode_headers key=k item=head}
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							{if $head == 'total_bases'}
								<th class = "numeric-sort" id="th_id_{$th_id}">{($sample_results.$head)|number_format:0:',':' '}</th>
							{elseif $head == 'nb_reads'}
								<th class = "numeric-sort"  id="th_id_{$th_id}">{($sample_results.$head)|number_format:0:',':' '}</th>
							{elseif $head == 'median_read_length'}
								<th class = "numeric-sort"  id="th_id_{$th_id}">{($sample_results.$head)|number_format:0:',':' '}</th>
							{elseif $head == 'median_read_quality'}
								<th class = "numeric-sort"  id="th_id_{$th_id}">{($sample_results.$head)|number_format:1:',':' '}</th>
							{elseif $head == 'N50_read_length'}
								<th class = "numeric-sort"  id="th_id_{$th_id}">{($sample_results.$head)|number_format:0:',':' '}</th>
							{elseif $head == 'L50_read_length'}
								<th class = "numeric-sort"  id="th_id_{$th_id}">{($sample_results.$head)|number_format:0:',':' '}</th>
							{elseif $head == 'barcode_score'}
								<th class = "numeric-sort"  id="th_id_{$th_id}">{($sample_results.$head)|number_format:1:',':' '}</th>
							{elseif $head == 'nb_read_Q>10'}
								<th class = "numeric-sort"  id="th_id_{$th_id}">{($sample_results.$head)|number_format:0:',':' '}</th>
							{elseif $head == 'total_bases_Q>10'}
								<th class = "numeric-sort"  id="th_id_{$th_id}">{($sample_results.$head)|number_format:0:',':' '}</th>
							{elseif $head == 'median_yield_per_sec'}
								<th class = "numeric-sort"  id="th_id_{$th_id}">{($sample_results.$head)|number_format:0:',':' '}</th>
							{elseif $head == 'nb_actif_channel'}
								<th class = "numeric-sort"  id="th_id_{$th_id}">{($sample_results.$head)|number_format:0:',':' '}</th>
							{/if}
							{$th_id = $th_id +1}
							
							{if $head == 'Reads'}
								<td id="sample_{$i}_col_{$col_id}">{$sample_results[$sample].$head|number_format:0:',':' '}</td>
								{$totalReads = $totalReads + $sample_results[$sample].$head}
							{elseif $head == 'Bases'}
								<td id="sample_{$i}_col_{$col_id}">{$sample_results[$sample].$head|number_format:0:',':' '}</td>
								{$totalBases = $totalBases + $sample_results[$sample].$head}
							{/if}
							
							{$col_id = $col_id + 1}
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						{/foreach}
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					</tr>
					{$i = $i + 1}
          		{/if}
            {/foreach}

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		</tbody>
	   	
	   	<tfoot>
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			<tr>	
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				<th>Total : </th>
				<th>&nbsp;</th>
				{foreach from=$barcode_headers key=k item=head}
					{if $head == 'Reads'}
						<th>{$totalReads|number_format:0:',':' '}</th>
					{elseif $head == 'Bases'}
						<th>{$totalBases|number_format:0:',':' '}</th>
					{/if}
				{/foreach}
			</tr>
			<tr>
				<th>Mean : </th>
				<th>&nbsp;</th>
				{foreach from=$barcode_headers key=k item=head}
					{if $head == 'Reads'}
						<th>{($totalReads/$nb_samples)|number_format:0:' ':' '}</th>
					{elseif $head == 'Bases'}
						<th>{($totalBases/$nb_samples)|number_format:0:' ':' '}</th>
					{/if}
				{/foreach}
			</tr>
			<tr>
				<th>All metrics :  <input type="checkbox" id="check_all_metrics"></th>
				<th>&nbsp;</th>
				{$th_id = 2}
				{foreach from=$barcode_headers key=k item=head}
					<th><center><input type="checkbox" id="chk_col_{$th_id}"></center></th>
					{$th_id = $th_id +1}
				{/foreach}
			</tr>
			<tr>
				<th align="left" colspan="{$th_id}">
					With selection :
					<button type="button" class="btn btn-default multiple-selection-btn" id="create_graph"><i class="glyphicon glyphicon-signal"></i> Compare</button>
				</th>
			</tr>
		</tfoot>
	</table>
	
	{/if}

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	<div class="tx-nG6-pi1-help">
		<img src="" alt="" class="img" />
		<p>Help for Basic metrics report :</p>
		<span class="meta">
			<ul>
				<li><strong>nb_reads</strong> : 
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					The total number of reads for this Flow Cell (including the reads that are filtered during the basecalling).
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				</li>
				<li><strong>total_bases</strong> : 
					The number of bases for this Flow Cell.
				</li>
				<li><strong>median_read_length</strong> : 
					The median length of the total reads (bases).
				</li>
				<li><strong>N50_read_length</strong> : 
					50% of all bases come from reads longer than this value.
				</li>
				<li><strong>median_yield_per_sec</strong> : 
					The median number of bases per second for the pores of this Flow Cell.
				</li>
				<li><strong>nb_actif_channel</strong> : 
					The number of activ channels during the sequencing.
				</li>
			</ul>
		</span>
	</div>
	<div class="tx-nG6-pi1-help">
		<img src="" alt="" class="img" />
		<p>Help for Quality report :</p>
		<span class="meta">
			<ul>
				<li><strong>median_read_quality</strong> : 
					The median quality (qscore ONT) for this Flow Cell.
				</li>
				<li><strong>nb_read_Q>N</strong> : 
					The total number of reads that have a quality > N.
				</li>
				<li><strong>total_bases_Q>N</strong> : 
					The total number of bases for the reads that have a quality > N.
				</li>
			</ul>
		</span>
	</div>
	
	<div class="tx-nG6-pi1-help">
		<img src="" alt="" class="img" />
		<p>Help for Plots report :</p>
		<span class="meta">
			<ul>
				<li><strong>cumulyieldperhour</strong> : 
					The cumulate number of bases per hour for this Flow Cell.
				</li>
				<li><strong>outrm_</strong> : 
					The outsiders have been removed.
				</li>
				<li><strong>outrm_distriblength</strong> : 
					The length distribution of reads for this Flow Cell.
				</li>
				<li><strong>outrm_distribqscore</strong> : 
					The quality (qscore ONT) distribution of reads for this Flow Cell.
				</li>
				<li><strong>outrm_lengthvsqscore_density</strong> : 
					The length and qscore distribution of reads with density for this Flow Cell.
				</li>
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				{if $barcode_name_count > 1 }
				<li><strong>qscoreboxplot</strong> : 
					Boxplots for each barcode of the mean qscore of reads for this Flow Cell.
				</li>
				<li><strong>qscorepertimeintervalsboxplot</strong> : 
					Boxplots for each barcode of the mean qscore of reads per interval of time for this Flow Cell.
				</li>
				<li><strong>qscorevariation</strong> : 
					Variation per time for each barcode of the mean qscore of reads for this Flow Cell.
				</li>
				{/if}
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			</ul>
		</span>
	</div>
{/block}