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GAFL
Pipelines_Snakemake
wgs_gatk
Commits
3fcc5a3d
Commit
3fcc5a3d
authored
May 21, 2021
by
Jacques Lagnel
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update README
parent
ad9a0638
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README.md
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### 2) reads QC, fastq report
### 3) merges overlaping read and keep unmerged Paired end (R1 & R2)
### 4) mapping with bwa,
a)
m
aps unmerged reads (PE)
b)
m
aps merged reads
c)
m
erge the 2 bam file
a)
M
aps unmerged reads (PE)
b)
M
aps merged reads
c)
M
erge the 2 bam file
### 5) mapping QC report (multiqc)
### 6) SNPs calling using GATK in parallel mode: reference splitted by sequences: #tasks=#samples X #chrs
####
a) GATK using "Best Practices Workflows" https://gatk.broadinstitute.org/hc/en-us/sections/360007226651-Best-Practices-Workflows
####
b)
u
sing hard filtering as outlined in GATK docs
####
https://gatkforums.broadinstitute.org/gatk/discussion/2806/howto-apply-hard-filters-to-a-call-set
####
https://gatk.broadinstitute.org/hc/en-us/articles/360035890471-Hard-filtering-germline-short-variants
a) GATK using "Best Practices Workflows" https://gatk.broadinstitute.org/hc/en-us/sections/360007226651-Best-Practices-Workflows
b)
U
sing hard filtering as outlined in GATK docs
https://gatkforums.broadinstitute.org/gatk/discussion/2806/howto-apply-hard-filters-to-a-call-set
https://gatk.broadinstitute.org/hc/en-us/articles/360035890471-Hard-filtering-germline-short-variants
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