DADA2 is a pipeline based on dada2 (Callahan et al., 2016) R package which consists of a series of steps to filter the raw sequences obtained through Illumina sequencing. The final step aims to obtain the taxonomy of the sequences that have been filtered in order to study the microbial community.
DADA2 is a Snakemake workflow based on dada2 (Callahan and al., 2016) and FROGS (Escudié and al, 2018) method which consists of a series of steps to filter the raw sequences obtained through Illumina sequencing. The final step aims to obtain the taxonomy of the sequences that have been filtered in order to study the microbial community.
Running DADA2 consists on calling a single command from the command line.
The output data format include biom format, phyloseq objets and R data sets.
## Getting ready
First we clone the git repository, in your line command go to your working directory and past the line below.